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Environmental Engineering Research 2005;10(2): 79-87. DOI: https://doi.org/10.4491/eer.2005.10.2.079
Seung-Bong Lee1, and Geonha Kim2
1ENVITECH, LLC, Redmond, 16541 Redmond Way #303 Redmond WA 98052WA, USA
2Department of Civil and Environmental Engineering, Hannam University, Ojungdong, Daedukku,Daejon, 306-791, Korea
Corresponding Author: Seung-Bong Lee ,Tel: +1-425-890-3510, Fax: +1-425+898-8236 , Email: sakelee@envi-technology.com
Received: May 8, 2003;  Accepted: April 10, 2005.
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The effects of transformation capacity on cometabolic degradation of trichloroethene (TCE) were evaluated using TCE-degrading actinomycetes pure and mixed culture under various culture conditions. The TCE transformation capacity of the actinomycetes enrichment culture in a batch test with phenol addition was 1.0 mg of TCE/mg of volatile suspended solids (VSS). The resting cell TCE transformation capacity of the actinomycetes pure culture cell was 0.75 mg TCE/mg VSS, which increased to 2.0 mg TCE/mg VSS when phenol was added as an external substrate. When the pure culture had an internal substrate in the form of poly-/?-hydroxybutyrate (PHB) at 19% of the cell mass, the resting cell TCE transformation capacity increased from 0.47 to 0.6 mg TCE/mg VSS. The presence of PEQ3 increased transformation capacity by 57%, whereas, the addition of phenol caused more than two fold increase in transformation capacity. The actinomycetes culture showed the highest transformation capacity.
Keywords: transformation capacity | actinomycetes | TCE
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