| Analysis of Total Bacteria, Enteric Members of γ-proteobacteria and
Microbial Communities in Seawater as Indirect Indicators for
Quantifying Biofouling |
| Jinwook Lee1, Sung-Min Kim1, Ji-Yeon Jung1, Byung Soo Oh2, In S. Kim2†, and Soon Kang Hong3 |
1Bio-Environmental Engineering Lab. (BEEL), Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju 500-712, Republic of Korea
2Center for Seawater Desalination Plant, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju 500-712, Republic of Korea
3Department of Fire Service Administration, Chodang University, 419 Sungnamri, Muan, Chonnam 534-701, Korea |
Corresponding Author:
In S. Kim ,Tel: +82-62-970-3382, Fax: +82-62-970-2434, Email: iskim@gist.ac.kr
|
|
Received: July 13, 2008; Accepted: February 16, 2009. |
|
|
Share : 
|
| ABSTRACT |
|
In this study, total bacteria, enteric members of the γ-proteobacteria, and microbial communities in seawater were analyzed as indirect indicators
for quantifying biofouling. Biomass in seawater can significantly affect feed water pretreatment and membrane biofouling of reverse osmosis desalination
processes. The purpose of this paper is to investigate microbiological quantity and quality of seawater at the potential intake of a desalination
plant. For this analysis, the total direct cell count (TDC) using 4'-6-diamidino-2-phenylindole (DAPI)-staining and DNA-based real-time PCR were
used to quantify the total bacteria and relative content of enteric members of γ-proteobacteria in seawater, respectively. In addition, microbial communities
were examined using 16S rRNA gene cloning and bacterial isolation to identify the most abundant bacteria for a further biofouling study.
The experimental results of this study identified about 106 cells/mL of (total) bacteria, 105 16S rRNA gene copies/mL of enteric γ-proteobacteria,
and the presence of more than 20 groups of bacteria. |
| Keywords:
Seawater | Biomass | Microbial community | Biofouling | Membrane |
|
|
|
PDF Links
Full text via DOI
Download Citation 
