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Environmental Engineering Research 2009;14(1): 19-25. DOI: https://doi.org/10.4491/eer.2009.14.1.019
Analysis of Total Bacteria, Enteric Members of γ-proteobacteria and Microbial Communities in Seawater as Indirect Indicators for Quantifying Biofouling
Jinwook Lee1, Sung-Min Kim1, Ji-Yeon Jung1, Byung Soo Oh2, In S. Kim2, and Soon Kang Hong3
1Bio-Environmental Engineering Lab. (BEEL), Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju 500-712, Republic of Korea
2Center for Seawater Desalination Plant, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju 500-712, Republic of Korea
3Department of Fire Service Administration, Chodang University, 419 Sungnamri, Muan, Chonnam 534-701, Korea
Corresponding Author: In S. Kim ,Tel: +82-62-970-3382, Fax: +82-62-970-2434, Email: iskim@gist.ac.kr
Received: July 13, 2008;  Accepted: February 16, 2009.
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ABSTRACT
In this study, total bacteria, enteric members of the γ-proteobacteria, and microbial communities in seawater were analyzed as indirect indicators for quantifying biofouling. Biomass in seawater can significantly affect feed water pretreatment and membrane biofouling of reverse osmosis desalination processes. The purpose of this paper is to investigate microbiological quantity and quality of seawater at the potential intake of a desalination plant. For this analysis, the total direct cell count (TDC) using 4'-6-diamidino-2-phenylindole (DAPI)-staining and DNA-based real-time PCR were used to quantify the total bacteria and relative content of enteric members of γ-proteobacteria in seawater, respectively. In addition, microbial communities were examined using 16S rRNA gene cloning and bacterial isolation to identify the most abundant bacteria for a further biofouling study. The experimental results of this study identified about 106 cells/mL of (total) bacteria, 105 16S rRNA gene copies/mL of enteric γ-proteobacteria, and the presence of more than 20 groups of bacteria.
Keywords: Seawater | Biomass | Microbial community | Biofouling | Membrane
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