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DOI: https://doi.org/10.4491/eer.2021.135
A mixed culture performing nitrite-dependent anaerobic methane oxidation and the nitrite removal mechanism revealed by high-throughput sequencing
Han Yan1,2, Jianzheng Li1,2, Jia Meng1,2, Xin Wang1,2, Lianggang Tang1,2, and Avinash Kumar Jha1,2
1State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, PR China
2School of Environment, Harbin Institute of Technology, Harbin, 150090, PR China
Corresponding Author: Jianzheng Li ,Tel: +86-451-86283761, Fax: +86-451-86283761, Email: jianzhengli@hit.edu.cn
Received: March 15, 2021;  Accepted: July 8, 2021.
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ABSTRACT
Nitrite-dependent anaerobic methane oxidation (n-DAMO) has been regarded as a promising effective approach to nitrogen removal from wastewater. However, n-DAMO bacteria are very difficult to be enriched in biological wastewater treatment processes. An anaerobic sequencing batch reactor (AnSBR) was introduced in the present study for the enrichment of n-DAMO bacteria with cornfield soil as inoculum. Fed with nitrite (NO2−) and methane as the specific substrates, a NO2− load removal as high as 46.16 mg/(L·d) was obtained in the AnSBR since the 232nd day of enrichment culturing, though the relative abundance of Candidatus Methylomirabilis referring to n-DAMO bacteria was 2.37% in the acclimatized mixed culture. High-throughput sequencing of the obtained mixed culture revealed that the community structure was complex with the coexistence of n-DAMO bacteria, methanotrophs, heterotrophic denitrifying bacteria and hydrolytic fermentation bacteria. Analysis of interactions among the prevalent microbial populations suggested that Candidatus Methylomirabilis had played a key role in the metabolic network of the mixed culture. The research work presented a novel approach to the enrichment of n-DAMO bacteria from cornfield soil and was helpful in understanding the role of n-DAMO bacteria in complex matrices.
Keywords: Community structure | Methanotrophs | Mixed culture | Nitrite-dependent anaerobic methane oxidation (n-DAMO) | Nitrite removal
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