Augmentation of the coagulation activity of alum using a porous bio-flocculant for the remediation of trihalomethanes-generating hydrophobic natural organic matter
Article information
Abstract
Conventional coagulation techniques have shown inefficacy for the removal of DBPs precursor i.e. hydrophobic NOM. The present study has used a bio-flocculant named Ficus benghalensis to enhance the coagulation activity of alum for the removal of hydrophobic NOM. The coagulation activity of the augmented treatment (Alum-F. benghalensis) system was validated using spectroscopic analysis. The augmented system had shown an increment in DOC and A254 removal by ~30% and ~16%, respectively. ASI and A272 had shown a continual decrease (from 0.12 to 0.08 and 0.46 to 0.23, respectively) for the augmented system. Chlorine demand was found lesser for the augmented system (1.4 mg/L) compared to alum (1.55 mg/L) only. The augmented system had shown a drastic reduction (from 0.1459 to 0.0638) in the value of (-ΔA272), which is directly related to the reduction in THMFP. The combined effects of sweep coagulation and charge neutralization were the responsible mechanisms for the augmented coagulation system. The noteworthy anti-bacterial activity of F. benghalensis was observed due to the presence of silver, copper, and traces of chlorine in its structure. Thus, the proposed augmented coagulation system can be used to remove the hydrophobic NOM from the water.
1. Introduction
The occurrence of natural organic matter (NOM) in the surface water due to the disposal of surface runoff, soil leachate, agricultural waste, industrial waste, and domestic sewage has increased the organic load in surface water [1–3]. NOM is defined as heterogeneous mixture of humic (hydrophobic/aromatic) and non-humic (less hydrophobic/aliphatic) organic components [4]. The hydrophobic fraction of NOM has shown its reactivity towards disinfectants such as chlorine, ozone, chlorine dioxide to form carcinogenic and genotoxic disinfectant by-products [5–7]. According to literature, hydrophobic fractions (humic acid and fulvic acid) of NOM have a prominent contribution to dissolved organic carbon (DOC) in water. These DOC in water will form disinfectant by-products (DBPs) such as trihalomethanes (THMs), haloacetonitriles (HANS), haloketones (HKs), chloral hydrate (CH), chloropicrin (CP), and halo acetic acid (HAA) in chlorinated water [8–10].
Literature depicts that NOM goes through substitution/oxidation reaction (during chlorination) with hypochlorous acid (HOCl) to form organic-halide compounds like THM and HAA [11–17]. THM is one of the most prominent DBPs in surface water, as it has adverse carcinogenic and non-carcinogenic risks and detrimental effects on human health as well as it adds about 20% of total DBPs [10, 18–20].
Coagulation and adsorption are effective remediation approach for the removal of THMs surrogates, aromatic NOM, in order to control the chlorinated by-products formation in drinking water [21]. Coagulation is described as “the destabilization of negatively charged colloidal and suspended particles dispersed in water using coagulating agents”, has been considered as suitable technique for the removal of high molecular weight as well as negatively charged colloidal particles from water [21]. These coagulating agents have been categorized as metallic salts (alum, ferric salts, zirconium oxychloride, and titanium tetrachloride), synthetic organic polymers (polyaluminium chloride), natural coagulants (Castenea Sativa, Moringa oleifera, Strychnos potatorum, and Cassia jahavikai), natural polymers (corn starch, paddy starch, elastin, and collagen), and natural polymer derivatives chitosan (N-deacetylated derivative of chitin) [10, 22–29]. The intricate structure, partial biodegradation, and low solubility of synthetic organic polymers have made it unsuitable as a coagulant [22, 30]. Even though, alum has been the most preferred coagulant across the globe owing to its low cost, easy availability, and its less sludge production than lime and ferrous salt, but it might exhibit few disadvantages like increment in dissolved solids, overdosing causes human risks like Alzheimer’s disease, the formation of huge amounts of sludge compares to natural coagulant, and less removal of NOM [23].
The extracts of natural coagulants are being used nowadays as bio-flocculant to enhance the coagulation activity of the inorganic coagulants. Although, the use of natural coagulants is economical and effective remediation approach for the treatment of high turbid water [25, 26]. But, the organic matrix of biomass tissues, microorganisms, and animal extracts such as polysaccharides, protein, and nucleic acid tends to increase the organic load in water [31]. The low coagulation activity of natural coagulants in the reduction of turbidity of low turbid water has been validated by several researchers [26]. Even the removal efficiency of natural coagulants can be raised by reducing organic contents from its extract by the ion-exchange process [32]. Still, it is considered as a practical approach for field application. Due to organic nature and low coagulation activity of natural coagulant in low turbid water, a new approach, i.e., enhanced coagulation (or augmented coagulation), could be used to study the variation in characteristics of colloidal impurities dispersed in water.
In recent years, augmented coagulation has proved a well-optimized process as it has increased the removal efficiency of inorganic coagulants. The mutual effects of sweep coagulation and precipitate charge neutralization are the responsible mechanisms for the augmented coagulation [33]. Generally, bio-flocculant [i.e., Moringa oleifera, Cyamopsis tetragonoloba] causes an increment in total organic carbon (TOC) whereas, TOC depends upon molecular weight and the active component of organic carbon. ASI and A272 have a linear relation with DOC and active aromatic groups, respectively, that are responsible for trihalomethanes formation potential (THMFP) [10, 34–36] and showed a continual decrease from the conventional coagulation to augmented coagulation process.
The noteworthy research gaps for the current study are the limitations of alum as a coagulant (i.e., increment in dissolved solids, overdosing causes Alzheimer’s disease, huge sludge production than natural coagulants, etc.) for the removal of aromatic NOM (surrogates of THMs), low coagulation activity of natural coagulants while its suitability as bio-flocculant to treat THM’s surrogates, assessment of THMFP using spectral indices, etc. These gaps are addressed in the current study. Considering the limitations of alum and natural coagulants, an augmented system has been proposed, which showed better suitability for the removal of active aromatic NOM fraction and ultimate THMFP reduction.
In the present study, the coagulation activity of alum is augmented using a bio-flocculant named Ficus benghalensis (or F. benghalensis) for the removal of turbidity and NOM surrogates [DOC, UV-absorbance at 272 nm (A272), and specific UV-absorbance (SUVA)]. The effects of various influencing parameters on coagulation activity were also assessed. The NOM consumption by chlorination process was further validated using spectral indices (i.e., A272, ASI, and −ΔA272). The spectroscopy and microscopy analysis were carried out to get the physiochemical attributes of the coagulants. Anti-bacterial assay of F. benghalensis was also carried out to get the zone of inhibition, which corresponds to the presence of disinfectants like silver, copper, and chlorine in its structure. The proposed augmented coagulation system can be widely used to remove the hydrophobic NOM from water.
2. Experimental
2.1. Chemicals
Aluminum sulphate [Al2(SO4)3.18H2O] commonly known as “alum”, sodium hypochlorite (NaOCl), sodium hydroxide (NaOH), hydrochloric acid (HCl), and starch were purchased from Merck, India. Humic acid and kaolin were purchased from Loba Chemie, India. F. benghalensis seeds were supplied by Sun Seeds Pvt. Ltd., India. All the chemicals were of analytical grades.
2.2. Reagents Preparation
The alum solution was prepared by dissolving 1 g of alum in 1 L of deionized water. Synthetic water enriched with NOM was prepared using humic acid solution and kaolin as per the reported method [24, 37, 38]. The predetermined pH and turbidity of synthetic water were adjusted using 0.1 N HCl/NaOH and kaolin, respectively.
Collected seeds of F. benghalensis were dried and grounded into powder form using a blender. Active components of F. benghalensis were extracted by mixing 1 g of coagulant powder in 1 L of deionized water (Millipore, Germany). The solution was stirred at 20 rpm for 20 minutes in magnetic stirrer (Tarsons, India) [10]. Coagulant solution was further filtered through filter paper (42 No., Whatman Int. Ltd, England). Freshly prepared F. benghalensis solution was preserved and used for coagulation.
2.3. Cultural Media Preparation for the Anti-Bacterial Test
The set nutritional agar (HI media) petri plates were dried at 37°C in a laminar air flow chamber under UV light and inoculated with stock Escherichia coli ATCC 25922 (American Type Culture Collection, Rockville, MD). Stock Escherichia coli ATCC 25922 was used due to its suitability for antibiotic susceptibility assays. The inoculated plates had three 6 mm diameter cut-outs on Whatman filter paper into the surface of the agar. The cut-outs were dipped with F. benghalensis solution, and all plates were incubated (34°C) overnight [39]. After incubation, the zone of inhibition was measured by calculating its diameter with the help of calipers.
2.4. Jar Test
Jar test apparatus (Scientific Engineering Corp., India) was used for the coagulation experiment. During coagulation, synthetic water was placed in four paddled jars. For simple coagulation, alum was added, and the solution was subjected to rapid mixing at 200 rpm for 2 min, followed by slow mixing for 40 rpm for 15 min under suitable operational conditions. Whereas, for augmented coagulation, defined doses of alum added as a primary coagulant and allowed for rapid mixing, further a predetermined doses of F. benghalensis was added during slow mixing to increase the flocculation activity. Flocs were allowed to form by placing the sample in the steady state for 30 min. After settling of colloidal particles, the samples were collected with the help of a syringe in the plastic bottles for the quality analysis. The test conditions during the experimentation are given in Table 1.
2.5. Analysis
The measurement of pH, TOC, DOC, A254, SUVA, and chlorine demand was carried out as per the APHA standard protocols [40]. Zeta potential was analyzed by the zeta analyzer (Horiba Scientific, Nano Particle, Japan). TOC and DOC were measured using a high-temperature combustion infrared method (TOC analyzer, Shimadzu, Japan). Samples were filtered through 0.45 μm nitrocellulose membrane before DOC and spectral analysis. Turbidity and pH were measured using a Digital turbidity meter (E.I., India) and bench-scale pH meter (Hanna Instruments, Germany), respectively. Analysis of various spectral indices (A254, ASI, and A272) was conducted using a UV-Vis spectrophotometer (Lab-tech, China). The significance of different spectral indices has been shown in Table 2. Differential spectroscopy (-ΔA272) with reference to chlorine consumption for the treated water was carried out as per the reported method [41]. All the experiments were conducted in triplicate for reproducibility purposes.
X-ray diffraction (XRD) (Ultima IV, Rigaku, Japan) and Fourier transform infrared spectroscopy (FTIR) (Agilent Technology) analysis were conducted as per reported methods [42]. Similarly, morphological characteristics of the coagulant and agglomerates were examined using field-emission scanning electron microscopy (FE-SEM) (Zeiss, Germany). The anti-bacterial assay was conducted to study the disinfection property of the coagulant.
3. Results and Discussion
Although F. benghalensis is not an efficient coagulant, it is useful as a bio-flocculant. In this work, the main focal point is on augmented coagulation using F. benghalensis as bio-flocculant based on percentage removal of turbidity and NOM surrogates, and assessment of spectral indices. Before elaborating on the performance of F. benghalensis as a coagulant and bio-flocculant, the physiochemical characterization was carried out to know the chemical reactions involved during the coagulation process. The antibacterial activity of F. benghalensis was also assessed to validate the presence of disinfectants like silver, copper, and chlorine in it. The detailed study, including physiochemical properties and performance of the F. benghalensis as a bio-flocculant, has been discussed as follows.
3.1. Physiochemical Characterizations of Powdered Seeds and Sludge of F. benghalensis (bio-flocculant)
The speciation of functional groups present in F. benghalensis is shown in Fig. 1 (a). The spectral range of 1,600–1,800 cm−1 corresponds to the C=O bond stretching and carbonyl group present in the protein and fatty acid structures [10, 43, 44]. The peak at 1,610.27 cm−1 shows the presence of aromatic C-C and C-H bonds, and the peak at 2,925.48 cm−1 shows the symmetric and asymmetric stretching of the C-H group present in fatty acid [45]. The presence of carbonyl group, fatty acid, protein, and alkyl group validated that F. benghalensis contains organics in its matrix.
The X-ray diffractogram of F. benghalensis has been shown in Fig. 1 (b). The poorly resolved peaks indicate the amorphous nature due to the presence of protein, lipids, and unsaturated fatty acid in F. benghalensis. The crystalline region of coagulant is possibly due to diffraction of protein around the particles having a more amorphous structure; this behavior is evident since non-skewed F. benghalensis seed was used [46]. The XRD diffractograms show the presence of minerals such as Freedite (Pb8Cu(AsO3)2O3Cl5) and Jalpaite (Ag3CuS2). The presence of silver (Ag), copper (Cu), and Cl (chlorine) elicit anti-bacterial characteristics of F. benghalensis, which is further validated in the anti-bacterial study [9, 47, 48].
The FE-SEM micrograph of F. benghalensis powder and its agglomerates are shown in Fig. 1 (c), (d). The FE-SEM image of F. benghalensis represents the globular and porous structure. The honeycomb structure of F. benghalensis [Fig. 1 (d)] of treated agglomerate shows that the sweep coagulation as a possible mechanistic approach for F. benghalensis mediated coagulation process [49]. The FE-SEM analysis of alum-F. benghalensis (augmented coagulation) treated agglomerates are shown in Fig. 1 (e), which represents the dispersed flocs over the plate-like structure. These results suggest that the synchronized effects of charge neutralization and sweep coagulation are the driving force for augmented coagulation using alum-F. benghalensis [49].
3.2. Anti-Bacterial Activity of F. benghalensis Powder
In the present study, the anti-bacterial activity of F. benghalensis powder was carried out, and the result was compared with chlorine based on the zone of inhibition (Table 3).
The result suggetes that zone of inhibition increased linearly with concentration initially, but the diameter of the zone of inhibition decreased after 250 mg/L and 10 mg/L concentration for F. benghalensis and chlorine, respectively. These results showed a dip due to the increase in molecular distribution that yields several interactions in nearby polymeric chains. Lower concentration probably promotes higher mobility of moieties and availability of more charged sites for external coupling due to less interaction between polymeric chains [50]. But at higher concentration, hydrogen bonds and covalent bonds might be formed between the functional groups of F. benghalensis, leading to densely overlapping coils formation around it, which eventually restrict its interfacial interaction. Consequently, an inferior number of charged sites would be available for interaction, which could affect the binding to bacterial cell walls [51, 52]. Thus, it could be interpretated that high concentration of disinfectant might affect the zone of inhibition. Thus, the maximum inhibition concentration (MIC) will be 250 mg/L and 10 mg/L for F. benghalensis and chlorine, respectively. The significant anti-bacterial activity of F. benghalensis is due to the presence of various anti-bacterial agents like silver, copper, and chlorine in its structure, as discussed in XRD analysis. Similarly, Priya et al. [10] have reported the anti-bacterial activity of the natural coagulants, such as M. oleifera and C. tetragonoloba.
Based on the physiochemical and anti-bacterial study, it could be assumed that the F. benghalensis has the potential to act as bio-flocculant to augment the coagulation activity of the alum for the removal of hydrophobic NOM. In the subsequent sections, the performance of the augmented coagulation system has been tested for the removal of hydrophobic NOM and validated using spectral indices.
3.3. pH Optimization and Validation
The dependency of the coagulant activity of the augmented coagulation system on the pH of the water has been assessed at a constant coagulant dose (alum: 2 mg/L and F. benghalensis: 1 mg/L). Usually, slightly alkaline pH favors the flocculating activity of natural coagulants. But, in the case of F. benghalensis, it has shown high efficiency at pH 6, which may be due to the presence of oxide and sulfide of metals, as shown by the XRD analysis. Researchers have already validated that alum worked efficiently in slightly acidic conditions [53]. In the present study, it was observed that F. benghalensis and alum exhibited maximum removal of turbidity and NOM surrogates (i.e., TOC, DOC, A254, and SUVA) at pH 6 [Fig. 2 (a) and (b)], because the zeta potential is very close to zero at pH 6 [Fig. 2 (c)] for both alum and F. benghalensis, respectively. Results suggested that synchronized impact of alum (primary coagulant) and F. benghalensis (bioflocculant) exhibited optimum coagulant activity for the removal of the NOM surrogates (i.e., TOC, DOC, SUVA, and A254) and turbidity at pH 6.
Zeta analysis was performed to validate the optimum pH [Fig. 2 (c)] as a function of variation in surface charge of the colloidal particles. In Fig. 2 (c), zeta potential tend to decrease from pH 5 to 6 due to better agglomeration and then increased at higher pH values due to less removal of negatively charged colloidal particles. At pH 6, zeta potential values seemed very close to zero for both F. benghalensis and alum due to better removal of turbidity and NOM surrogates, so at this pH, the colloidal particles will be in stable condition as maximum particles get stabilized, suggesting pH 6 as optimized parameter.
3.4. Physiochemical Parameters of the Source Water
Synthetic water was prepared to have a resemblance to surface water enriched with aromatic NOM. The physiochemical characteristics of tap water and synthetic water are given in Table 4.
3.5. Performance Evaluation of Augmented Coagulation System
Performance evaluation of F. benghalensis as a bio-flocculant was assessed based on the removal of turbidity, NOM surrogates (i.e., DOC, A254, SUVA), and spectral indices (i.e., ASI, A272, −ΔA272) from samples. During the coagulation experiment for alum and F. benghalensis, the pH was constant at 6 pH, and the dose was 2 mg/L for alum and 1 mg/L for F. benghalensis. In the case of augmented coagulation, only the dose of F. benghalensis was varied in the range of 1–4 mg/L, and its effect on coagulation activity was assessed.
Although, the efficiency of coagulants to remove suspended colloidal particulates or turbidity is the preliminary parameter to assess their coagulant activity but, it might be insufficient to insight into the variation in NOM characteristics during coagulation. The removal of dispersed particles using F. benghalensis, alum, and augmented coagulation from low turbid water have been shown in Fig. 3 (a). Alum has shown higher turbidity removal (11.12%) than F. benghalensis (7.54%) in low turbid water, which corresponds to the reported literature [25, 26]. The presence of charged ions could have allowed alum to attract dispersed negatively charged suspended particles, while, F. benghalensis reduced turbidity through sweep coagulation. However, a slight improvement in the turbidity removal was observed, when F. benghalensis was used as a bio-flocculant along with alum (at a dose of 2 mg/L of alum and 4 mg/L of F. benghalensis), about 13.14% [Fig. 3 (b)]. Thus, it could be concluded that turbidity removal efficiency of augmented coagulation tend to be better at a higher dose of F. benghalensis due to the mutual effect of sweep coagulation and charge neutralization.
The increasing trend of total organic carbon in treated effluent using F. benghalensis as a coagulant and bio-flocculant has been shown in Fig. 3 (a) and (b). In the present study, the TOC removal efficiency of alum was estimated as 17.05%, but in the case of F. benghalensis and augmented coagulation, TOC has been increased by 0.36% and 1.85%, respectively, which could be due to higher dose of organic F. benghalensis (i.e., 4 mg/L in case of augmented coagulation). This minute increase in TOC could not be directly related to THMFP increment since this increment might be due to an increase in the hydrophilic fraction of NOM [54]. Similarly, Fooladvand et al. [55] have reported that with an increase in TOC from 3 to 7 mg/L, the THMFP has increased from 50.04 ± 5.53 to 164.14 ± 9.19 μg/L.
Dissolved organic carbon is one of the prominent criteria for the assessment of the aromatic fraction of NOM in water as shown in Fig. 3 (a). The concentration of DOC increased by 10.2% in the effluent treated with F. benghalensis as a coagulant, which is due to an organic matrix of coagulant. However, alum imparted DOC removal efficiency to some extent (~3.77%). A significant reduction in DOC was observed for augmented coagulation (~29.5%) at a dose of 2 mg/L of alum and 4 mg/L of F. benghalensis [Fig. 3 (b)]. In the case of augmented coagulation, the DOC removal was reduced with an increase in the dose of F. benghalensis, but at a higher dose, the DOC removal further increased. This result suggest that either alum will suppress the coagulant activity of F. benghalensis to reduce the DOC removal in treated water or combined effects of charge neutralization and sweep coagulation will support the DOC reduction. Similarly, Ramavandi et al. [56] have used DOC as an indicator for THM reduction.
Estimation of the UV absorbing fraction of NOM at the wavelength of 254 nm represents the extent of DOC and hydrophobic NOM present in water as shown in Fig. 3 (b) [24]. In the case of augmented coagulation, maximum removal of A254 absorbing materials was achieved (at the same dose of 2 mg/L alum and 4 mg/L F. benghalensis) as compared to F. benghalensis and alum. A254 removal efficiency is low for F. benghalensis treated water due to an increase in the dissolved portion, mainly hydrophilic fraction. Still, in the case of augmented coagulation, removal efficiency has been increased due to rise in flocculation activity by adding bio-flocculant that causes the mutual effect of sweep coagulation and precipitate charge neutralization. Priya et al. [24] have also used the A254 to show the removal of hydrophobic NOM, which has good correlation with DOC reduction.
SUVA (= 100*A254/DOC) represents the aromatic content, mainly hydrophobic fraction of NOM [24]. Fig. 3 (a) shows that SUVA reduction is more for F. benghalensis (11.21%) as compared to alum (3.61%) due to the removal of hydrophobic fractions. In the case of augmented coagulation system, SUVA value has increased by 16.36% [Fig. 3 (b)] due to more reduction in DOC value as compared to A254 value, there may also be the possibility of the reduction in the hydrophilic portion instead of a hydrophobic portion of DOC. It can be seen that SUVA value has been increased at all doses of alum and F. benghalensis.
Furthermore, the measurement of the coagulation activity of augmented coagulation system has been measured using spectral indices, as discussed below.
3.6. Spectral Indices
ASI represents the rate of changes of NOM absorbance as well as a reduction in DOC, which has a direct relation with THMFP and chlorine demand [34]. The ASI value also represents the apparent molecular weight fraction of NOM [10]. Fig. 3 (c) shows the declining trend of ASI from synthetic to treated water except for F. benghalensis treated water, and a lesser ASI value was observed for augmented coagulation. In the case of F. benghalensis, ASI has increased due to an increase in the hydrophilic fraction of DOC which validates the concept of augmented coagulation using aluminum sulphate and F. benghalensis for the control of trihalomethanes in chlorinated water.
A272 shows the presence of activated aromatic groups responsible for disinfectant by-products formation potential [35]. Fig. 3 (c) shows that the A272 value is continuously decreasing from synthetic to treated water, except for F. benghalensis treated water. A maximum reduction in A272 is observed for augmented coagulation system, so it can be stated that augmented coagulation is an efficient process to treat NOM enriched water. Similarly, Priya et al. [10] have used the spectral indices like ASI and A272 to show the reduction of active hydrophobic NOM fraction, which is responsible for the THMFP.
3.7. Chlorine Demand and Differential Spectroscopy (-ΔA272)
The chlorine demand was estimated at an optimized pH 6 as shown in Fig. 4 (a). The chlorine demand for synthetic water was estimated as 1.68 mg/L that is higher than the treated water, which could be due to the absence of anti-bacterial and disinfection agents. Fig. 4 (a), depicted that chlorine demand for F. benghalensis coagulated water (1.5 mg/L) is less than alum coagulated water (1.55 mg/L) due to the antibacterial activity of F. benghalensis. Least chlorine demand has been observed in augmented coagulation (1.4 mg/L), which might be due to the efficient removal of aromatic content as well as the antibacterial activity of F. benghalensis. The breakpoint chlorination curves for F. benghalensis, alum, and augmented coagulation has been shown in Fig. 4 (a).
Previous studies have demonstrated that differential spectroscopy (-ΔA272) has a definite correlation with DBPs formation on reaction with chlorine [41, 57]. Differential spectroscopy (-ΔA272) showing the chlorine consumption by treated water was performed as per the reported method [41]. From Fig. 4 (b), a linear relationship between chlorine doses and −ΔA272 was observed in the effluent treated with alum and F. benghalensis, respectively, as a well significant drop in −ΔA272 was observed for water treated using augmented coagulation system. That is mainly due to the combined effects of charge neutralization and sweep coagulation. F. benghalensis would promote higher rates of DBP formation as the value of −ΔA272 is increased than alum, possibly due to higher organic content in. F. benghalensis. But, the treatment of NOM enriched water using alum-F. benghalensis would mark a significant drop in DBP formation, which ascribes to the enhanced coagulation activity of the augmented coagulation system. Roccaro et al. [57] have also used differential spectroscopy (-ΔA272) to show the reduction of DBP formation potential, which has shown a good correlation with the DBPs reduction.
4. Conclusions
As, the direct removal of THMs is difficult, so the THM surrogates (i.e., aromatic NOM) has been targeted to be removed using coagulation process. Considering the inefficacy of the conventional coagulation process, an augmented coagulation technique has been proposed, in which the coagulation activity of the alum was increased using a novel bio-flocculant named F. benghalensis. The augmented coagulation system has shown a significant reduction of NOM surrogates (TOC, DOC, turbidity, A254, SUVA). The influence of pH and dose of coagulants was also correlated with the coagulation activity. The removal of aromatic NOM and subsequent THMFP reduction was further assessed by using spectral indices (ASI, A272, −ΔA272), which are in good agreement with the removal of NOM surrogates. The spectroscopy, microscopy, and anti-bacterial analysis of F. benghalensis has also shown the amorphous nature, porous structure, presence of organic matrix, and disinfecting agents in it. These are responsible for the enhanced removal efficiency of the augmented coagulation system, lesser chlorine demand, and reduction in THMFP. The proposed augmented coagulation system can be widely used for the remediation of hydrophobic NOM from water.
Acknowledgment
The study was carried out within the research project (SR/S3/CEE/00owledgement04/2013) funded by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), India. We would also like to thank the Department of Environmental Science and Engineering, Indian Institute of Technology (Indian School of Mines), Dhanbad, for providing financial support under the GATE fellowship funded by the Ministry of Human Resource Development (MHRD), the Government of India, Delhi.
Notes
Author Contributions
B.G. (M. Tech student) has performed the experimentation, analysis of water, physiochemical characterization of the coagulants and treated water, written the first draft of the manuscript, and finally reviewed and corrected the manuscript. T.P. (Ph.D. student) has helped in the experimentation, manuscript writing, and helped in reviewing and correcting the manuscript. B.K.M. (Assistant Professor) has supervised the work, helped in conceptualization, reviewed the manuscript and corrected it.