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DOI: https://doi.org/10.4491/eer.2018.040
Bacterial community analysis of stabilized soils in proximity to an exhausted mine
Jae Eun Park1, Byung-Tae Lee2, Byung-Yong Kim3, and Ahjeong Son1
1Department of Environmental Science and Engineering, Ewha Womans University, Seoul 03760, Republic of Korea
2Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61004, Republic of Korea
3Bioinformatcis Research Institute, Chun Lab, Inc., Seoul 06725, Republic of Korea
Corresponding Author: Ahjeong Son ,Tel: +82-2-3277-3339 , Fax: +82-2-3277-3275, Email: ahjeong.son@gmail.com
Received: January 22, 2018;  Accepted: April 13, 2018.
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ABSTRACT
Soil stabilization is a soil remediation technique that reduces the mobility of heavy metals in soils. Although it is a well-established technique, it is nonetheless essential to perform a follow-up chemical assessment via a leaching test to evaluate the immobilization of heavy metals in the soil matrix. Unfortunately, a standard chemical assessment is not sufficient for evaluation of the biological functional state of stabilized soils slated for agricultural use. Therefore, it is useful to employ a pyrosequencing-based microbial community analysis for the purpose. In this study, a recently stabilized site in the proximity of an exhausted mine was analyzed for bacterial diversity, richness, and relative abundance as well as the effect of environmental factors. Based on the Shannon and Chao1 indices and rarefaction curves, the results showed that the stabilized layer exhibited lower bacterial diversity than control soils. The prevalence of dominant bacterial populations was examined in a hierarchical manner. Relatively high abundances of Proteobacteria and Methylobacter tundripaludum were observed in the stabilized soil. In particular, there was substantial abundance of the Methylobacter genus, which is known for its association with heavy metal contamination. The study demonstrated the efficacy of (micro)biological assessment for aiding in the understanding and post-management of stabilized soils.
Keywords: Bacterial community analysis | Heavy metal | Methylobacter | Pyrosequencing | Soil stabilization
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