Measuring Phosphatase Activity in Peatland Soils:
Recent Methodological Advances |
Chris Freeman1, Inyoung Jang2, Kyoung duk Zho3, and Hojeong Kang2† |
1School of Biological Sciences, University of Wales, Bangor, UK 2School of Civil and Environmental Engineering, Yonsei University, Korea 3School of Public Health, Seoul National University, Korea |
Corresponding Author:
Hojeong Kang ,Tel: +82-2-2123-5803, Fax: +82-2-364-5300, Email: hj_kang@yonsei.ac.kr |
Received: December 1, 2008; Accepted: December 15, 2008. |
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ABSTRACT |
Measurements of phosphatase activity in peatlands are made difficult by the low levels of activity and the characteristically high concentrations
of dissolved organic matter within the sediments. These materials may cause high background absorbances in colorimetric assays, and quenching
interference in fluorimetric assays. This review describes the development of a new approach which allows such problems to be overcome by using
HPLC to separate the interferences from the products of enzymic hydrolysis. This approach is applicable to various environmental samples such as
peat, wetland sediment, and sludge which may contain a large amount of interfering organic matters. |
Keywords:
Methylumbelliferyl phosphate | HPLC | Quench correction | Organic soil |
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